Accumaxis a ready to use non-mammalian, non-bacterial replacement for all applications of trypsin and collagenase in tissue dissociation, cell counting, and dissolving cell clumps such as spheroids.
Accumaxcontains the same enzymes asAccutaseand is a direct replacement for collagenase.
Accumaxcan be used to increase the accuracy of a cell count. It can also be added to a clumpy sample on a cell sorter to extend the sort time of the sample.
Advantages of Accumax
Increases accuracy of manual or automated cell counts.
Used to dissolve neuronal and prostate spheroids.
Removes cells from 3-D matrixes.
Removes cells from hollow fiber cell reactors.
Extends the sort time of clumpy cell on a sorting flow cytometer.Cell Lines tested
A few cell lines thatAccumaxhas been shown to work on without harm:
hESCs, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.
Accumaxperforms exceptionally well for:
hESC culturings, analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogenetransfection, neural crest cell migration assays, cell proliferation, apoptosis, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Accumax is used to digest primary tissue, create single cell suspensions for cell counts, and declump cells for magnetic or flow cytometry cell sorting and removing from artificial growing matrixes.